MSCV-human Erbb2-IRES-GFP

LOCUS       MSCV-human Erbb2-IRES-GFP               10280 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Retroviral expression of human Erbb2.
ACCESSION   .
VERSION     .
KEYWORDS    MSCV-human Erbb2-IRES-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Vo BT, Li C, Morgan MA, Theurillat I, Finkelstein D, Wright S, Hyle 
            J, Smith SM, Fan Y, Wang YD, Wu G, Orr BA, Northcott PA, Shilatifard
            A, Sherr CJ, Roussel MF
  TITLE     Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive 
            Myc-Driven Group 3 Medulloblastoma.
  JOURNAL   Cell Rep. 2017 Mar 21;18(12):2907-2917. doi: 
            10.1016/j.celrep.2017.02.073.
  PUBMED    28329683
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             197..713
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    777..1118
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1185..1601
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1531..1553
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1569..1585
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5582..5599)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5809..5828
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5990..6709
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5990..6706
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6035..6056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6296..6315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6643..6664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6871..7385
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7547..7563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7571..7601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7616..7637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7754..7771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7925..8513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8005..8024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8684..9544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9307..9326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9545..9649)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9717..9735
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9773..9795)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9895..9914
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10108..10130
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10258..10277
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat
       61 tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat
      121 atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc
      181 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
      241 cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt
      301 aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
      361 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
      421 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
      481 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
      541 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg
      601 gtacccgtat tcccaataaa gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc
      661 cttgggaggg tctcctcaga ttgattgact gcccacctcg ggggtctttc atttggaggt
      721 tccaccgaga tttggagacc cctgcccagg gaccaccgac ccccccgccg ggaggtaagc
      781 tggccagcgg tcgtttcgtg tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat
      841 gtttgcgcct gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg
      901 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg
      961 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat
     1021 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     1081 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt
     1141 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     1201 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     1261 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     1321 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     1381 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     1441 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     1501 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     1561 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaattggg atcccagtgt
     1621 ggtggtacgt aggaattgat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc
     1681 ctcttgcccc ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg
     1741 ctccctgcca gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag
     1801 gtggtgcagg gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg
     1861 caggatatcc aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc
     1921 ccactgcaga ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg
     1981 gccgtgctag acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca
     2041 ggaggcctgc gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg
     2101 atccagcgga acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac
     2161 aagaacaacc agctggctct cacactgata gacaccaacc gctctcgggc ctgccacccc
     2221 tgttctccga tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc
     2281 ctgacgcgca ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac
     2341 tgctgccatg agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc
     2401 tgcctccact tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac
     2461 aacacagaca cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc
     2521 tgtgtgactg cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc
     2581 tgccccctgc acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc
     2641 agcaagccct gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg
     2701 gcagttacca gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg
     2761 gcatttctgc cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca
     2821 gagcagctcc aagtgtttga gactctggaa gagatcacag gttacctata catctcagca
     2881 tggccggaca gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga
     2941 attctgcaca atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg
     3001 ctgcgctcac tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc
     3061 tgcttcgtgc acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc
     3121 cacactgcca accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg
     3181 tgcgcccgag ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc
     3241 cttcggggcc aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat
     3301 gtgaatgcca ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg
     3361 acctgttttg gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc
     3421 ttctgcgtgg cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg
     3481 aagtttccag atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt
     3541 gtggacctgg atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc
     3601 atctctgcgg tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc
     3661 atcaagcgac ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg
     3721 gagctggtgg agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc
     3781 ctgaaagaga cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc
     3841 tacaagggca tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg
     3901 ttgagggaaa acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg
     3961 gctggtgtgg gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg
     4021 cagctggtga cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc
     4081 ggacgcctgg gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc
     4141 tacctggagg atgtgcggct cgtacacagg gacttggccg ctcggaacgt gctggtcaag
     4201 agtcccaacc atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag
     4261 acagagtacc atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt
     4321 ctccgccggc ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag
     4381 ctgatgactt ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg
     4441 ctggaaaagg gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc
     4501 atggtcaaat gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct
     4561 gaattctccc gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg
     4621 ggcccagcca gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg
     4681 ggggacctgg tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac
     4741 cctgccccgg gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt
     4801 ggcggtgggg acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca
     4861 ctggcaccct ccgaaggggc tggctccgat gtatttgatg gtgacctggg aatgggggca
     4921 gccaaggggc tgcaaagcct ccccacacat gaccccagcc ctctacagcg gtacagtgag
     4981 gaccccacag tacccctgcc ctctgagact gatggctacg ttgcccccct gacctgcagc
     5041 ccccagcctg aatatgtgaa ccagccagat gttcggcccc agcccccttc gccccgagag
     5101 ggccctctgc ctgctgcccg acctgctggt gccactctgg aaaggcccaa gactctctcc
     5161 ccagggaaga atggggtcgt caaagacgtt tttgcctttg ggggtgccgt ggagaacccc
     5221 gagtacttga caccccaggg aggagctgcc cctcagcccc accctcctcc tgccttcagc
     5281 ccagccttcg acaacctcta ttactgggac caggacccac cagagcgggg ggctccaccc
     5341 agcaccttca aagggacacc tacggcagag aacccagagt acctgggtct ggacgtgcca
     5401 gtgtgactcg agcgggatca attccgcccc ccccctaacg ttactggccg aagccgcttg
     5461 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5521 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5581 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5641 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5701 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5761 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5821 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5881 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5941 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggtgagcaa
     6001 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6061 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6121 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6181 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6241 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6301 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6361 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6421 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6481 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6541 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6601 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6661 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     6721 ctctagagtc gacctgcagg catgcaagct tcaggtagcc ggctaacgtt aacaaccggt
     6781 acctctagaa ctatagctag catgcgcaaa tttaaagcgc tgatatcgat aaaataaaag
     6841 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     6901 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     6961 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     7021 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     7081 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     7141 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     7201 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     7261 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     7321 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     7381 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     7441 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     7501 tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     7561 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7621 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7681 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9301 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9361 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9421 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9481 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9541 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9601 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9661 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     9721 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9781 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9841 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9901 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     9961 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
    10021 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    10081 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggcgcaag
    10141 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
    10201 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
    10261 gatgtcggcg atataggcgc
//